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Cell culture Porcine aortic endothelial cells (PAECs) were isolated as described 45 and cultured at 37 C, 5% CO 2 , and 80% humidity in DMEM containing 10% heat-inactivated fetal calf serum (FCS), penicillin (100 U/ml), streptomycin (0.1 mg/ml), and amphotericin (1.25 g/ml)

The viral inoculum concentration for each leaf was 50 L of 20 g/mL