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This neutralization is achieved through a process called glutathione conjugation, catalyzed by a superfamily of enzymes known as Glutathione S-Transferases (GSTs)

Although the reaction mixture was prepared under anaerobic conditions, catalase was added to ensure that if reduced SmTGR were to react with any available dioxygen, the resulting H 2 O 2 would quickly be disproportionated and moreover, this activity further depletes oxygen with the sample

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When the loop is disrupted by heavy oxidative demand, certain medications, or specific enzyme deficiencies pyroglutamic acid can accumulate, a state clinicians call pyroglutamic acidemia or 5-oxoprolinuria