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Cells were incubated with a mouse monoclonal antibody targeting the human transferrin receptor (Clone H68.4, #136800, Invitrogen, Eugene, OR) diluted to a final concentration of 2 g/mL in PBS overnight at 4 C

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The supplement also improved two functional biomarkers in urine, FIGLU and methylmalonic acid, indicating the supplement decreased the need for folic acid and vitamin B12, respectively

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