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BioTechniques 21, 418422

Lactating women, at least 18 years of age, with a history of Plasmodium vivax infection, with no previous primaquine treatment for radical cure, with a currently breastfeeding infant at least 28 days of age, and provide informed consent were recruited to the study

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Cells were then washed with PBS, fixed with cold 1:1 methanol and acetone for 3 min and permeabilized with 0.2% Triton X-100 for 5 min