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Metabolic effects of a growth hormonereleasing factor in patients with HIV. NEJM , 2007

The process involves inserting a needle through the vial's rubber stopper and slowly adding bacteriostatic water to the lyophilized peptide

And size and location are the key factors

7.5.3 Impurities (United States Pharmacopeia, 1985) 7.5.3.1 Preparation of sodium nitrite to test Sodium nitrite (1 g) is dissolved in 6 ml hydrochloric acid (3 mol/l) and then evaporated to dryness on a steam bath