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All animals were housed under specific pathogen-free conditions at the TIGEM animal facility (Pozzuoli, Italy) with 12-h light/dark cycles and received food and water ad libitum with Cu in diet at 8 g/kg concentration (Cat, #FRDBA 10-10

We were suddenly thrown into a world of IVs, antibiotics, steroids, pain meds, blood draws, and round-the-clock monitoring

References Stavoe AKH, Holzbaur ELF (2019) Autophagy in neurons

The Science Behind "Leaky Gut"/Intestinal Permeability The intestinal barrier -- which is a mere 1 cell-width wide (stop and think about how small that is for a second!) -- consists of tight junctions, which are complex, specialized structures that regulate what passes between the gut and the bloodstream

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