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To complement transcript-level data, spatial proteomics techniques like imaging mass cytometry (IMC), cyclic immunofluorescence (cycIF), co-detection by indexing (CODEX), iterative bleaching extends multiplexity (IBEX), and multiplexed ion beam imaging (MIBI) allow simultaneous mapping of dozens of protein markers across individual cells in situ, which reveals how immune and stromal cell phenotypes and cellcell interactions vary spatially [164]

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