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Decompensation was mostly characterized by varices, ascites, and hepatic encephalopathy

SAF2017-88108-R, PID2020-114977RB-I00, RTC2019-006825-1, PDC2021-121883-I0, RED2022-134786-T, funded by MICIU/AEI /10.13039/5011000110330 and by MICIU/AEI /10.13039/501100011033 and Next GenerationEU/ PRTR, AGAUR (2017SGR-323, 2021SGR-720), Fundaci TV3-La Marat (202030), and la Caixa Foundation (ID 100010434), under the agreement LCF/PR/HR20/52400018

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When intestinal microecology is disrupted, LPS enters blood circulation, activates the Toll-like receptor 4 (TLR4) signaling, and upregulates the expression of the NF-B pathway, which in turn promotes the differentiation and activity of osteoclasts, leading to severe bone loss and osteoporosis (62)

As a result, the immobilized cells on loofah sponge could be reused five times over a period of 526 h, and lag phase was only detected in the first batch and the fermentation period was significantly shortened, increasing the yield and productivity of -PL from 22.53 g/L and 3.30 g/L/day to 34.11 g/L and 9.34 g/L/day, respectively [106]