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To interrogate the mechanism of LDE detoxification impairment in test conditions, (i) sublethal L-buthionine sulfoximine (BSO) and (ii) sodium orthovanadate are used to generate standard curves for inhibition of only the first or second steps of the LDE detoxification pathway, respectively ( Figure 1C )

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For IP, cells were lysed in NETN lysis buffer (150 mM NaCl, 1 mM EDTA, 10 mM Tris-HCl, pH 8.0, 0.5% Igepal, and fresh protease inhibitor cocktail) as described previously 47

suggested that intraperitoneal injection of extracellular cold-inducible RNA-binding protein (eCIRP) reduced the expression of GPX4 in lung tissue and increased lipid ROS, while ferrostatin-1 treatment significantly reduced this increase [68]

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