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Shoots were excised at about 1.0 cm down from the apical tip and cultured on rooting medium (MS medium supplemented with 0.1 mg/L NAA, 0.1 g/L Tim, 0.1 g/L Cefo, and 4.5 mg/L hygromycin (for GFP transformants) or 50 mg/L kanamycin (for GUS transformants)) at 25 C and 16 h light/8 h light/dark cycles

Exposure to secondhand smoke can increase the risk of cancer and other diseases

We were provided with cheap brown ones, with wooden stems, which were much liked by the natives, and it is probable that small brier-wood pipes, which are not liable to break, would form an acceptable addition to any stock of trading goods"

He perfected the blend for a new cigarette known as Lucky Strike, which was later to become one of the leading brands in the history of the industry