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Calcium ions as second messengers in guard cell signal transduction

Phosphate solubilization activity was measured by inoculating bacterial isolates into 100 mL of National Botanical Research Institutes Phosphate (NBRIP) broth, which contained the following components per liter: 10 g glucose, 5 g Ca 3 (PO 4 ) 2 , 5 g MgCl 2 6H 2 O, 0.25 g MgSO 4 7H 2 O, 0.2 g KCl, and 0.1 g (NH 4 ) 2 SO 4 along with 0%, 5%, or 10% PEG6000 (pH 7) 55