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Total RNA was extracted with the high pure RNA isolation kit, and the total RNA was transcribed into cDNA using the All-in-one First Strand cDNA Synthesis Kit

These same time points analyzed by Western blot showed that while robust adduct formation was possible, formation of GDAP1-4HNE adducts was not immediate but rather lagged far behind binding

cGMP-elevating compounds and ischemic conditioning provide cardioprotection against ischemia and reperfusion injury via cardiomyocyte-specific BK channels

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