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Optimization of gradient and HPLC-MS/MS conditions The presence of various polar functional groups, such as carboxylic, amine, or thiol groups, in the structures of the analytes, makes them suitable for analysis in HILIC mode on polar stationary phases

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Available human studies are characterized by relatively small sample sizes, heterogeneity in dosing regimens, and short intervention durations, which restrict the ability to draw firm conclusions regarding efficacy and optimal therapeutic windows

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