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Medesan C, Cianga P, Mummert M, Stanescu D, Ghetie V, Ward ES

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Briefly, protein extracts were prepared by homogenizing with a Polytron homogenizer (KINEMATICA AG, Switzerland) in lysis buffer containing: 20 mM HEPES (pH 7.9), 25% v/v glycerol, 0.42 M NaCl, 0.2 mM EDTA, 1.5 mM MgCl2, 0.5% v/v Nonidet P-40, 1 mM DTT, 1 mM Naf, 1 mM Na 3 VO 4 , and 1 complete protease inhibitor cocktail (Roche Diagnostics Ltd, Mannheim, Germany)

Therefore, DHODH/CoQH 2 and mitochondrial GPX4 constitute two major ferroptosis defense systems to eliminate cell damage or death caused by the accumulation of mitochondrial lipid peroxides, and preclinical studies have shown that targeting DHODH can promote ferroptosis[36]

Acta Orthop Scand 70:487490 Yeler H, Tahtabas F, Candan F (2005) Investigation of oxidative stress during fracture healing in the rats