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In the context where immune checkpoint blockade (ICB) has demonstrated remarkable efficacy in cancer immunotherapy, several studies have revealed the reduced effectiveness of anti-PD-1/PD-L1 therapy in aged mice and elderly patients, 207,208,209 leading to treatment resistance

828,829 In addition, iSAT enables the study and engineering of ribosomes that are competent for translation but unable to support life in laboratory conditions

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Subsequently, the cells were collected, embedded in epoxy resin and sliced with a thickness of 5070 nm

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