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The pellet containing the mitochondrial fraction was resuspended in incubation buffer (120 mM KCl, 10 mM Tris, 5 mM KH 2 PO 4 , adjusted to pH 7.4 with HCl)

The results showed that although there were no significant changes in hormones, there was a significant decrease in exercise induced muscle damage

Xu Y, et al

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Similarly, exercise elevates metabolism, causing an increase in the use of oxygen and eventually resulting in oxidative stress 16 , 17