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Endocrinol Metab Clin North Am

Journal of Cell Biology , 204 (5), 669682

To introduce the C-terminal Azo-tag into the coding region for the sfGFP reporter protein, two Gly codons and the amber stop codon were inserted between the C-terminal Strep -tag II and the final ochre stop codon, thus placing Pap as the last encoded residue in the reading frame, and this gene cassette was subcloned on pSB19 between the Xba I and Hin dIII restriction sites

However, recent studies have shown that the improvement of inhibition depends on TMAO, which should be able to minimize the risk of thrombosis (Roberts et al., 2018)