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doi: 10.1016/j.ucl.2005.08.007 63 BurnettALMusickiB

To view a copy of this licence, visit About this article Cite this article Monteiro, J.P.P., dos Santos, C.C.M., de Queiroz, J.P.M

In addition, cells in mature biofilms exhibit altered gene expression patterns, with up-regulation of drug efflux pumps and stress-response pathways (heat-shock proteins, calcineurin signaling), metabolic shifts resulting in micro-niches (areas with hypoxia or nutrient limitation) that diminish activity from fungicidal drugs (Ramage et al., 2005

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Briefly, the thermocycling protocol followed a pre-incubation at 95C for 10 minutes, followed by 45 cycles of 3-Step amplification: 1) denature at 95C for 20 seconds